Mastichochoria is a big part of Chios. They are at the southern part of Chios and they are well known for the production of mastic. As of 2018 there are 24 Mastichochoria: Mesta, Olimpi, Pirgi, Armolia, Kalamoti, Patrika, Kini, Vouno, Nenita, Katarraktis, Kallimassia, Messa Didima, Ekso Didima, Mirmigi, Tholopotami, Agios Georgios, Vessa, Elata, Lithi, Neochorio, Flatsia, Pagida, Thimiana, Vavili.
Mastic production in Chios is protected by a Europian Union protected designation of origin. In 1938, the Chios Mastic Growers Association was founded as a secondary cooperative organisation that acts as the representative of twenty primary cooperatives founded in the twenty-four Mastichochoria.
Interesting fact, in the Chios Massacre of 1822, during the Ottoman rule of Chios, the people of Mastichochoria were spared so that they could continue serve the Sultan by providing him and his harem with mastic.